Stephen mc grath and douwe van sinderen department. In his roles as director, president, and chancellor, watson led cshl to articulate its presentday mission, dedication to exploring molecular biology and genetics in order to advance the understanding and ability to diagnose and treat cancers, neurological diseases, and other causes of human suffering. Bacteriophages, the viruses that parasitize and kill bacteria, can be used in several different types of genetic analysis. This volume is a survey of the genetics of bacteria and their viruses, and it provides students with a comprehensive introduction to this rapidly changing subject. However, formatting rules can vary widely between applications and fields of interest or study. The scientist who introduced the name bacteriophage was felix dherelle, who investigated these viruses for many years, leading to new fields of research, including bacteriophage therapy. Working with bacteriophages and nematodes, the university of california, berkeley, molecular biologist uncovered. Molecular genetics of bacteria, 5th edition microbiology. Role of bacteriophages in genetics and molecular biology. The book is a sequel to the 1983 edition and is organized into six major sections. Bacteriophage download ebook pdf, epub, tuebl, mobi. Genetics and molecular biology inglese copertina rigida 1 lug 2007.
Written in the highly successful methods in molecular biology. Also included are many important papers on structural biology and molecular genetics and papers reflecting the initial developments in dna cloning and sequencing. Bacteriophages are composed of proteins that encapsulate a dna or rna genome, and may have structures that are either simple. In part 2, he discusses the organization of bacteriophage genomes and explains how genomic sequence comparison can provide information about viral evolution. The contributions of george beadle and edward tatum. Written by eminent international researchers actively involved in the disparate areas of bacteriophage research this book focuses on the current rapid developments in this exciting field. A cistrans complementation test defines a cistron, which is a gene.
Seymour benzer used the riilocus of phage t4 to define genes by virtue of their behavior in a complementation test, and also to provide fundamental insight into the structure of genes in particular, the arrangement of mutable sites see the next section. Jun 24, 2019 much of what we know about genetic fine structure, prior to the advent of techniques for isolating and sequencing genes, derive form studies in bacteriophage. Of value to all biologists with an interest in the molecular basis of living systems, the book is a personal selection by the editor. The propensity of bacteriophage to recombine and the ease of measuring this recombination were crucial during the explosive period when studies of bacteriophage largely created the new field of molecular biology, as portrayed in the excellent book phage and the origins of molecular biology cairns et al. The book is written for upper level undergraduates and beginning graduate students, particularly those who have had an introductory genetics course. Methods and protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject.
Much of the founding work in molecular biology and structural biology was done on bacteriophages. One was phage and the origins of molecular biology, a collection of articles honoring max delbrucks 60th birthday in 1966. Written by eminent international researchers actively involved in the disparate areas of bacteriophage research, this book focuses on the current rapid developments in this exciting field. They are minute particles virions with a protein covering capsid protecting a nucleic acid genome. Understanding an organism minus an individual component, and understanding the individual components minus the organism, genetics and biochemistry. Bacteriophage bioinformatics and genomics bacteriophages have played and continue to play a key role in bacterial genetics and molecular biology. Also the first genome to be sequenced was a bacteriophage. Like viruses that infect eukaryotes plants, animals, and fungi there are many different phage structures and functions phages are typically made of an outer protein hull that has genetic material inside it. Bacteriophage genetics bacteriophages, the viruses that parasitize and kill bacteria, can be used in several different types of genetic analysis.
The term is commonly shortened to phage bacteriophages are among the most common and diverse entities in the biosphere. Bacterial genetics and bacteriophages molecular and cell. Watson, crick and maurice wilkins were awarded the 1962 nobel prize in physiology or medicine for their discoveries concerning the molecular structure of nucleic acids and its. Everyday low prices and free delivery on eligible orders.
Genetics and molecular biology 1 by mcgrath, stephen, van sinderen, douwe isbn. Molecular biology of bacteriophage t4 highlights the value of this biological system as a research and teaching tool. This volume is a survey of the genetics of bacteria and their viruses, and it. Chapter 2 bacteriophage bioinformatics and genomics carlos a. In 1917, dherelle published the first paper describing the phenomenon of the bacteriophage and its biological nature. Bacterial and bacteriophage genetics request pdf researchgate. Bacteriophage genetics modern genetic analysis ncbi bookshelf. Bacteriophage simple english wikipedia, the free encyclopedia.
This book consists of 12 chapters highlighting the current rapid developments in the field of bacteriophage research. In 1953, he coauthored with francis crick the academic paper proposing the double helix structure of the dna molecule. Acm has opted to expose the complete list rather than only correct and linked references. Phage can confer key phenotypes on their host, for example converting a nonpathogenic strain into a pathogen, and they. Bacteriophage genetics an introduction to genetic analysis.
First, two distinct phage genotypes can be crossed to measure recombination and hence map the viral genome. Bacterial and bacteriophage genetics 4th edn heredity. P hage or bacteriophage is a virus that infects bacteria. James dewey watson born april 6, 1928 is an american molecular biologist, geneticist and zoologist. Since there is no preface, the editors goal can only be assumed from the contents of the book. This edition offers a greater emphasis on molecular biology and genetics than was present in either of. Genetics and molecular biology hardback and a great selection of related books, art and collectibles available now at. Canchaya, marco ventura, and douwe van sinderen bacteriophages have played and continue to play a key role in bacterial genetics and molecular biology. The book opens with an excellent chapter that provides a broad overview of the topics and also highlights the multifaceted nature of bacteriophage research. They are minute particles virions with a protein covering capsid. A, also known informally as a phage, is a virus that infects and replicates within bacteria and archaea. It starts with a chapter providing a broad overview of the topics and highlights the multifaceted nature of bacteriophage research. The molecular biology of viruses is a collection of manuscripts presented at the third annual international symposium of the molecular biology of viruses, held in the university of alberta, canada on june 2730, 1966, sponsored by the faculty of medicine of the university of alberta. Part of the methods in molecular biology book series mimb, volume 501 log in to check access.
Like viruses that infect eukaryotes plants, animals, and fungi there are many different phage structures and functions. Many of these tools underpin the past 50 years of molecular biology, and others. Oclcs webjunction has pulled together information and resources to assist library staff as they consider how to handle coronavirus. This engaging book is the first full biography of dherelle, a complex figure who. Reliable information about the coronavirus covid19 is available from the world health organization current situation, international travel. This book apart from the numerous texts recently published in the area of bacteriophage biology is the focus on the methodology used in this field.
The focus of the book remains firmly on bacteria and will be invaluable to students studying microbiology, biotechnology, molecular biology, biochemistry, genetics and related biomedical sciences. In the following years, bacteriophages became important model organisms in molecular biology and genetics. Ocr errors may be found in this reference list extracted from the full text article. Bacteriophage genetics modern genetic analysis ncbi. I read again my article in the book e dgar 1966 about the discovery of conditional lethals. Bacteriophage genetics modern microbial genetics wiley. Synopsis bacteriophages viruses that infect bacteria are fascinating organisms that have played and continue to play a key role in bacterial genetics and molecular biology. Bacteriophage have been a powerful model genetic system, because they have small genomes, have a short life cycle, and produce many progeny from an infected cell. In part 1, hatfull describes what bacteriophage are, some of their biological properties and how they were discovered. The term was derived from bacteria and the greek phagein, meaning to devour. Even in the absence of other phage genes, it is active in vivo 48, but its. This volume focuses largely on the classical protocols as described by the editors. Written in the highly successful methods in molecular biology series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a notes section which details tips on troubleshooting and avoiding known pitfalls.
Bacteriophages phages, or bacterial viruses, have been major research tools for molecular biology. A series of more than 110 articles and 6 major books followed, in which dherelle established the foundation for the later work of the phage group in molecular biology. During infection, a phage attaches to a bacterium and injects its genetic material into the bacterial cytoplasm figure 719. Oct 01, 2004 when i left the university of californiasanta cruz in 1990 and cleared out my office, i had saved a few scientific books. Bacteriophages were first described and named by felix dherelle in 1917 see dherelle, felix. Bacteriophages viruses that infect bacteria are fascinating organisms that have played and continue to play a key role in bacterial genetics and molecular biology. The phage genetic information then takes over the machinery of the bacterial cell by turning off the synthesis of bacterial components and redirecting the bacterial synthetic material to make more phage components figure 720. This book reveals their compelling elegence of function and their almost inconceivable diversity. Bacteriophages viruses that infect bacteria are fascinating organisms that have played and continue to play a key role in bacterial genetics and molecular. Oct 23, 2019 before beginning the formal study of the genetics of bacteria and their viruses, it is important to understand the fundamentals of a microbial cell, chromosome, and dna structures, and the basic viral processes. All of the major topics in modern bacterial and bacteriophage genetics are presented, among them mutations and mutagenesis, genetics of t4 bacteriophage and other intemperate and temperate phages, transduction, transformation, conjugation and plasmids.
Bacteriophages have played and continue to play a key role in bacterial genetics and molecular biology. Phage can confer key phenotypes on their host, for example converting a nonpathogenic strain into a pathogen, and they play a key role in regulating bacterial populations in all sorts of environments. From the beginning, dherelle was interested in the possibility of using phages. Bacterial and bacteriophage genetics 4th edn heredity nature. The book is written for upper level undergraduates and beginning graduate. Bacteriophage genetics an overview sciencedirect topics. Numerous and frequentlyupdated resource results are available from this search.
The state of genetics in this period was described in a textbook by alfred sturtevant and george beadle, an introduction to genetics. The recombination of bacteriophage genetic markers was first observed in. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. When i left the university of californiasanta cruz in 1990 and cleared out my office, i had saved a few scientific books. These are widely used in molecular biology research and in biotechnology, as probes and markers, and in the popular method of assesing gene expression. Request pdf bacterial and bacteriophage genetics genetic investigations and. Mutations arise in the absence of selection luria and delbruck 1943. Dna metabolism, regulation of gene expression, phage morphogenesis, structure and function of selected proteins, hostphage interactions, and experiments in t4 molecular genetics. About this textbook genetic investigations and manipulations of bacteria and bacteriophage have made vital contributions to our basic understanding of living cells and to the development of molecular biology and biotechnology. Different altered forms of these phages are used by molecular biologists in dna cloning, where they are convenient vectors because of their infectious nature. In the 1940s, and onward, it was the laboratory study of phage biology that directly yielded major insights into bacterial genetics, molecular biology, and the exact manner in which viruses reproduce and spread.
Jim watson has written a whole book about the discovery of the. The propensity of bacteriophage to recombine and the ease of measuring this recombination were crucial during the explosive period when studies of bacteriophage largely created the new field of molecular biology, as portrayed in the excellent book phage and the. Essentials of molecular genetics has been written with the objective of providing concise but complete knowledge on the abovementioned aspects of the chemical b asis of life, genetic material. Biotechnology tools derived from the bacteriophagebacteria arms. Before beginning the formal study of the genetics of bacteria and their viruses, it is important to understand the fundamentals of a microbial cell, chromosome, and dna structures, and the basic viral processes. Historically, they were used to define gene structure and gene regulation.
416 1305 358 604 33 168 48 921 494 709 1202 1326 1236 1509 940 7 369 1246 360 1240 174 731 1560 382 1276 1421 467 1149 549 281 149 1144 1360 1440 1228 1232 354 146 766 1360 1113 1390